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A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns.

Cell. 2018-07; 
GlassDavid S, Riedel-KruseIngm
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Peptide Synthesis … 170 Peptides. EPEA and PEAE peptides were synthesized by Genscript at > 95% purity. The lyophilized peptides were resuspended in water, and their concentration was quantified on a NanoDrop One using the A205/31 method. Aggregation time lapses …Blots were then incubated on a rotor in 3mL TBS-TM + 3 mL goat anti-GAPDH (Genscript #A00191-40) + 3 mL rabbit anticamelid VHH (Genscript #A01860-200). Get A Quote
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摘要

Synthetic multicellular systems hold promise as models for understanding natural development of biofilms and higher organisms and as tools for engineering complex multi-component metabolic pathways and materials. However, such efforts require tools to adhere cells into defined morphologies and patterns, and these tools are currently lacking. Here, we report a 100% genetically encoded synthetic platform for modular cell-cell adhesion in Escherichia coli, which provides control over multicellular self-assembly. Adhesive selectivity is provided by a library of outer membrane-displayed nanobodies and antigens with orthogonal intra-library specificities, while affinity is controlled by intrinsic adhesin ... More

关键词

adhesion,morphology,multicellularity,nanobodies,patterning,self-assembly,self-organization,synthetic bio