凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation.

Nat Commun. 2019-01; 
CampagneAntoine,LeeMing-Kang,ZielinskiDina,MichaudAudrey,Le CorreStéphanie,DingliFlorent,ChenHong,ShahidianLara Z,VassilevIvaylo,ServantNicolas,LoewDamarys,PasmantEric,Postel-VinaySophie,WassefMichel,MargueronRaph
Products/Services Used Details Operation
Molecular Biology Reagents alpha-Tubulin (1/3000 dilution; 1F4E3; A01410) was purchased from GenScript. Get A Quote

摘要

In Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAP1-associated core complex (BAP1.com) containing one of the redundant ASXL proteins. We investigate the mechanism underlying BAP1.com-mediated trans... More

关键词