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A Novel Biotinylated Lipid Raft Reporter For Electron Microscopic Imaging Of Plasma Membrane Microdomains.

J Lipid Res.. 2012-10;  53(10):2214 - 2225
Kimberly J. Krager, Mitul Sarkar, Erik C. Twait, Nancy L. Lill, and John G. Koland. Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA.
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摘要

The submicroscopic spatial organization of cell surface receptors and plasma membrane signaling molecules is readily characterized by electron microscopy (EM) via immunogold labeling of plasma membrane sheets. Although various signaling molecules have been seen to segregate within plasma membrane microdomains, the biochemical identity of these microdomains and the factors affecting their formation are largely unknown. Lipid rafts are envisioned as submicron membrane subdomains of liquid ordered structure with differing lipid and protein constituents that define their specific varieties. To facilitate EM investigation of inner leaflet lipid rafts and the localization of membrane proteins therein, a unique geneti... More

关键词

epidermal growth factor receptor; myristoylation; palmitoylation; metabolic biotinylation