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CHRAC/ACF contribute to the repressive ground state of chromatin.

Life Sci Alliance. 2018; 
Scacchetti Alessandro,Brueckner Laura,Jain Dhawal,Schauer Tamas,Zhang Xu,Schnorrer Frank,van Steensel Bas,Straub Tobias,Becker Pet
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Catalog Antibody were incubated overnight at RT with primary antibodies αV5 (1:1,000, GenScript, Cat. No. A00623 Get A Quote

摘要

The chromatin remodeling complexes chromatin accessibility complex and ATP-utilizing chromatin assembly and remodeling factor (ACF) combine the ATPase ISWI with the signature subunit ACF1. These enzymes catalyze well-studied nucleosome sliding reactions in vitro, but how their actions affect physiological gene expression remains unclear. Here, we explored the influence of chromatin accessibility complex/ACF on transcription by using complementary gain- and loss-of-function approaches. Targeting ACF1 to multiple reporter genes inserted at many different genomic locations revealed a context-dependent inactivation of poorly transcribed reporters in repressive chromatin. Accordingly, single-embryo transcript... More

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