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Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin Motors.

Curr. Biol.. 2017; 
Reinemann Dana N,Sturgill Emma G,Das Dibyendu Kumar,Degen Miriam Steiner,V?r?s Zsuzsanna,Hwang Wonmuk,Ohi Ryoma,Lang Matth
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摘要

During cell division, the mitotic kinesin-5 Eg5 generates most of the force required to separate centrosomes during spindle assembly. However, Kif15, another mitotic kinesin, can replace Eg5 function, permitting mammalian cells to acquire resistance to Eg5 poisons. Unlike Eg5, the mechanism by which Kif15 generates centrosome separation forces is unknown. Here we investigated the mechanical properties and force generation capacity of Kif15 at the single-molecule level using optical tweezers. We found that the non-motor microtubule-binding tail domain interacts with the microtubule's E-hook tail with a rupture force higher than the stall force of the motor. This allows Kif15 dimers to productively an... More

关键词

Eg5,Kif15,kinesin,kinesin-12,mitosis,mitotic motor,optical trap,single molecule,spindle asse