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Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation.

J. Agric. Food Chem.. 2019; 
ZhangYumin,SunQichao,ZhangChunxia,HaoGuanglong,WangChunmei,DirkLynnette M A,DownieA Bruce,ZhaoTian
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Proteins, Expression, Isolation and Analysis His-ZmABI5 protein was purified using a GenScript High Affinity Ni-Charged Resin (L00250) according to the 11 ACS Paragon Plus Environment Journal of Agricultural and Food Chemistry Page 12 of 34 231 manufacturer’s protocol. Get A Quote

摘要

Raffinose, an oligosaccharide found in many seeds, plays an important role in seed vigor; however, the regulatory mechanism governing raffinose biosynthesis remains unclear. We report here that maize W22 wild type (WT) seeds, but not W22 viviparous1 ( zmvp1) mutant seeds, start accumulating galactinol and raffinose 28 days after pollination (DAP). Transcriptome analysis of the zmvp1 embryo showed that the expression of GALACTINOL SYNTHASE2 ( GOLS2) was down-regulated relative to WT. Further experiments showed that the expression of ZmGOLS2 was up-regulated by ZmABI5 but not by ZmVP1, and it was further increased by the coexpression of ZmABI5 and ZmVP1 in maize protoplasts. ZmABI5 interacted with ZmV... More

关键词

ABI5,GALACTINOL SYNTHASE,maize,raffinose biosynthesis,seeds,vivipa