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Translational Regulation of Non-autonomous Mitochondrial Stress Response Promotes Longevity.

Cell Rep. 2019; 
LanJianfeng,RollinsJarod A,ZangXiao,WuDi,ZouLina,WangZi,YeChang,WuZixing,KapahiPankaj,RogersAric N,Ch
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Recombinant Antibody Services elegans RNAi Library (Ahringer) Source BioScience 3318_Cel_RNAi_complete Chemicals, Peptides, and Recombinant Proteins Taq DNA Polymerase ABM Cat# G008 T4 ligase ABM Cat# EL0011 Ligation-Free Cloning Kit ABM Cat# E002 Ampicillin BBI Cat# AB0028 Peptone BD Cat# 211677 Agar BD Cat# 214010 Tryptone BD Cat# 211705 Yeast Extract BD Cat# 212750 Agar BD Cat# 214010 Biodlight Western Chemiluminescent HRP substrate Bioworld Cat# BLH02S050 Proteinase K Fermentas Cat# EO0491 FastAP Thermosensitive Alkaline Phosphatase Fermentas Cat# EF0654 ExpressPlus PAGE Gel GenScript Cat# M42015C Tris-MOPS-SDS Running Buffer GenScript Cat# M00138 PVDF Immobilon Cat# IPVH00010 cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat# 4693132001 PhosSTOP Roche Cat# 4906845001 dNTP Sangon Cat# D0056 Ethidium bromide Sangon Cat# EX328 Sodium chloride Sangon Cat# A100241 Sodium dodecyl sulfate (SDS) Sangon Cat# S0227 EDTA Sangon Cat# EB0185 Tris (Base) Sangon Cat# TT1492 Potassium chloride Sangon Cat# A501212-0500 Magnesium sulfate anhydrous (MgSO4) Sangon Cat# A601988-0250 Calcium chloride (CaCl2) Sangon Cat# 10035-04-8 Isopropyl-b-D-thiogalactoside (IPTG) Sangon Cat# IB0168 Potassium phosphate dibasic (K2HPO4) Sangon Cat# PT1212 Potassium phosphate monobasic (KH2PO4) Sangon Cat# A600445 (Continued on next page) Cell Reports 28, 1050–1062.... Samples were boiled for 10 minutes before resolving on precast SDS-PAGE gels (GenScript). Get A Quote

摘要

Reduced mRNA translation delays aging, but the underlying mechanisms remain underexplored. Mutations in both DAF-2 (IGF-1 receptor) and RSKS-1 (ribosomal S6 kinase/S6K) cause synergistic lifespan extension in C.?elegans. To understand the roles of translational regulation in this process, we performed polysomal profiling and identified translationally regulated ribosomal and cytochrome c (CYC-2.1) genes as key mediators of longevity. cyc-2.1 knockdown significantly extends lifespan by activating the intestinal mitochondrial unfolded protein response (UPR), mitochondrial fission, and AMP-activated kinase (AMPK). The germline serves as the key tissue for cyc-2.1 to regulate lifespan, and germline-specif... More

关键词

AMPK,C.?elegans,UPR(mt),aging,daf-2 rsks-1,germline,mRNA transla