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Glycoprotein G (gG) production profile during infectious laryngotracheitis virus (ILTV) infection

PLoS One.. 2019; 
Bendezu J1, Morales Ruiz S1, Montesinos R1, Choque Guevara R1, Rojas-Neyra A1, Pauyac-Antezana K1, Fernández-Díaz M1.
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摘要

Glycoprotein G (gG) is a conserved protein, and it has been described as a chemokine-binding protein in most members of the alphaherpesviruses. In case of the infectious laryngotracheitis virus (ILTV), an alphaherpesvirus that infects chickens, this protein is a virulence factor that plays an immunomodulatory role in the chicken immune response. Nevertheless, the gG production profile during ILTV infection has not yet been studied. In this study, we developed monoclonal antibodies in order to determine the gG production profile during ILTV infection in chicken hepatocellular carcinoma (LMH) cell cultures as well as embryonated specific-pathogen-free (SPF) chicken eggs and SPF chickens using a sandwich enzyme-li... More

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