凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Nup96 and HOS1 are Mutually Stabilized and Gate CONSTANS Protein Level, Conferring Long-day Photoperiodic Flowering Regulation in Arabidopsis

Plant Cell.. 2019; 
Cheng Z1, Zhang X2, Huang P1, Huang G3, Zhu J1, Chen F2, Miao Y4, Liu L5, Fu Y6, Wang X2.
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis … the proteins were eluted with 2xSDS-PAGE loading buffer by heating at 95°C for 5 min, and<br> separated on 4-20% SurePAGEᵀᴹ precast gel (<b>Genscript</b>, M00657) followed by immunoblot<br> analyses … precast gel (8% SurePAGEᵀᴹ precast gel, M00663, <b>Genscript</b>, China) … Get A Quote

摘要

Lines of evidence indicate that nuclear pore complex profoundly affects the timing of plant flowering, but the underlying mechanisms remain poorly elucidated. Here, we report that Nucleoporin 96 (Nup96) acts as a negative regulator of long-day photoperiodic flowering in Arabidopsis. Through multiple approaches, we identified and verified an E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1) interacting in vivo with Nup96. Nup96 and HOS1 proteins mainly localize and interact on nuclear membrane. Loss-of-function of Nup96 leads to destruction of HOS1 proteins without changing its mRNA abundance, which results in over-accumulation of the key activator of long-day photoperiodic flowering, C... More

关键词