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IGFBP6 controls the expansion of chemoresistant glioblastoma through paracrine IGF2/IGF-1R signaling.

Cell Commun Signal. 2018; 
Oliva CR,,, Halloran B, Hjelmeland AB, Vazquez A,, Bailey SM, Sarkaria JN, Griguer CE,.
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To generate the wt-IGFBP6 (Swiss-Prot accession number P24592), a cDNA encoding full-length IGFBP6 cDNA was cloned into the pET-28a(+) expression vector (GenScript, Piscataway, NJ) using NdeI and XhoI restric- tion sites.... After incuba- tion, the membranes were washed, blocked for 1 h, and incubated with biotinylated NWSHPQFEK Tag antibody (Genscript #A01737, dilution 1:1000).

摘要

Glioblastomas (GBMs), the most common and most lethal of the primary brain tumors, are characterized by marked intra-tumor heterogeneity. Several studies have suggested that within these tumors a restricted population of chemoresistant glioma cells is responsible for recurrence. However, the gene expression patterns underlying chemoresistance are largely unknown. Numerous efforts have been made to block IGF-1R signaling pathway in GBM. However, those therapies have been repeatedly unsuccessful. This failure may not only be due to the complexity of IGF receptor signaling, but also due to complex cell-cell interactions in the tumor mass. We hypothesized that differential expression of proteins in the insulin-like... More

关键词

Brain tumor; Chemoresistance; Glioblastoma; IGF-1R; IGF2; IGFBP6; Temozolomide