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Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo.

J Cell Physiol. 2019; 
Thomas AM,, Dong Y, Beskid NM,, García AJ,, Adams AB, Babensee JE,.
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Catalog Antibody Splenocytes (5 × 106 cells/ml) were separated from red blood cells via a 5 min incubation in lysis buffer at room temperature, primed against ovalbumin, OVA257‐264/323‐229 (5 μg/ml each in basal media; Genscript; RP10610/RP10611) antigen for 6 days, and labeled with carboxyfluorescein succinimidyl ester (CFSE) (0. Get A Quote

摘要

Dendritic cells are key players in regulating immunity. These cells both activate and inhibit the immune response depending on their cellular environment. Their response to hyperglycemia, a condition common amongst diabetics wherein glucose is abnormally elevated, remains to be elucidated. In this study, the phenotype and immune response of dendritic cells exposed to hyperglycemia were characterized in vitro and in vivo using the streptozotocin-induced diabetes model. Dendritic cells were shown to be sensitive to hyperglycemia both during and after differentiation from bone marrow precursor cells. Dendritic cell behavior under hyperglycemic conditions was found to vary by phenotype, among which, tolerogenic den... More

关键词

dendritic cells; diabetes; hydrogel; hyperglycemia; immunity; interleukin-10