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D2-Like Dopamine And β-Adrenergic Receptors Form A Signaling Complex That Integrates Gs- And Gi-Mediated Regulation Of Adenylyl Cyclase.

Cell Signal.. 2012-11;  24(11):2051-60
Rebois RV, Maki K, Meeks JA, Fishman PH, Hébert TE, Northup JK. Laboratory of Cellular Biology, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, United States.
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摘要

β-Adrenergic receptors (βAR) and D2-like dopamine receptors (which include D2-, D3- and D4-dopamine receptors) activate Gs and Gi, the stimulatory and inhibitory heterotrimeric G proteins, respectively, which in turn regulate the activity of adenylyl cyclase (AC). β2-Adrenergic receptors (β2AR) and D4-dopamine receptors (D4DR) co-immunoprecipitated when co-expressed in HEK 293 cells, suggesting the existence of a signaling complex containing both receptors. In order to determine if these receptors are closely associated with each other, and with other components involved in G protein-mediated signal transduction, β2AR, D4DR, G protein subunits (Gαi1 and the Gβ1γ2 hetero... More

关键词

β-Adrenergic receptors; D2-like dopamine receptors; Heterotrimeric G proteins; Adenylyl cyclase; Signaling complexes; Bioluminescence resonance energy transfer; AlprenololCSepharose