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JMJD5 cleaves monomethylated histone H3 N-tail under DNA damaging stress.

EMBO Rep. 2017; 
Shen J, Xiang X, Chen L, Wang H, Wu L, Sun Y, Ma L, Gu X, Liu H, Wang L, Yu YN, Shao J, Huang C,, Chin YE,,.
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Catalog Antibody Serum was collected, purified, and tested for specificity as described in Fig EV5C; anti-K4me1-H3 (Abcam #8895), anti-K4me2-H3 (Abcam #7766), anti-pT6-H3 (Abcam #14102), anti-K9me0-H3 (Abcam #61251), anti-K9me1-H3 (Abcam #8896), anti-K9me2-H3 (Abcam #1220), anti-K9me3-H3 (Abcam #8898), anti-acK9-H3 (Abcam #4441), anti-pS10-H3 (Abcam #5176), anti-pT11-H3 (Abcam #5168), anti-K14me2-H3 (Upstate #07-427), anti-acK14-H3 (Abcam #52946), anti-K27me1-H3 (Upstate #07-448), anti-K27me2-H3 (Abcam #24684), anti-K36me1-H3 (Abcam #9048), anti-K79me2-H3 (Abcam #3594), anti-H3 C-term- inal region (Abcam #1791), anti-JMJD5 (Abcam #28883), anti-Myc (Santa Cruz Biotech #40), anti-H2A (CST #12349), anti-H2B (CST #2934), anti-H4 (Abcam #ab10158), a-GAPDH (MultiSciences #Mab5079), anti-GST (GenScript #A00865), anti-HA (Santa Cruz Biotech #7392), Alexa-680 or IRDye-800 goat anti-mouse or -rabbit secondary antibody (Li-COR). Get A Quote

摘要

The histone H3 N-terminal protein domain (N-tail) is regulated by multiple posttranslational modifications, including methylation, acetylation, phosphorylation, and by proteolytic cleavage. However, the mechanism underlying H3 N-tail proteolytic cleavage is largely elusive. Here, we report that JMJD5, a Jumonji C (JmjC) domain-containing protein, is a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions that cause a DNA damage response. JMJD5 clips the H3 N-tail at the carboxyl side of monomethyl-lysine (Kme1) residues. In vitro H3 peptide digestion reveals that JMJD5 exclusively cleaves Kme1 H3 peptides, while little or no cleavage effect of JMJD5 on dimethyl-l... More

关键词

H3K9me1; Histone H3; JMJD5; N‐tail cleavage