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The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity.

Cell Rep. 2018; 
Schultz-Larsen T, Lenk A, Kalinowska K, Vestergaard LK, Pedersen C, Isono E, Thordal-Christensen H.
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摘要

Plant "nucleotide-binding leucine-rich repeat" receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase "associated molecule with the SH3 domain of STAM3" (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1D505V and the catalytically inactive ESCRT-III protein SKD1E232Q in Nicotiana benthamiana supports the requirement of ESCRT-asso... More

关键词

AMSH3 deubiquitinase; ESCRT; autoimmunity; nucleotide-binding leucine-rich repeat receptors; plant immunity