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Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS.

Nat Microbiol. 2019; 
Ghosal D, Jeong KC,, Chang YW,, Gyore J, Teng L, Gardner A, Vogel JP, Jensen GJ,.
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摘要

Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). Here, we used complementary electron cryotomography and immunofluorescence microscopy to investigate the molecular architecture and biogenesis of the Dot/Icm secretion apparatus. Electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. Immunofluorescence microscopy deciphered an early-stage assembly pro... More

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