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A new MCM modification cycle regulates DNA replication initiation.

Nat Struct Mol Biol. 2016; 
Wei L,, Zhao X.
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Catalog Antibody Antibodies used were anti-HA (F-7, Sc-7392, Santa Cruz Biotechnology), anti-V5 (R960-25, Invitrogen), anti-myc (9E10, Bio X cell), PAP (P1291, Sigma), anti-flag (M2, Sigma), anti- Rad53 (yC-19, sc-6749, Santa Cruz Biotechnology), anti-Orc2 (SB46, Abcam), anti-Pgk1 (22C5D8, Invitrogen), anti-Cdc6 (9H8/5, Abcam), anti-Strep tag II (A01732, Genscript), anti-Psf1 and anti-Mcm6 (gifts from K. Get A Quote

摘要

The MCM DNA helicase is a central regulatory target during genome replication. MCM is kept inactive during G1, and it initiates replication after being activated in S phase. During this transition, the only known chemical change to MCM is the gain of multisite phosphorylation that promotes cofactor recruitment. Because replication initiation is intimately linked to multiple biological cues, additional changes to MCM can provide further regulatory points. Here, we describe a yeast MCM SUMOylation cycle that regulates replication. MCM subunits undergo SUMOylation upon loading at origins in G1 before MCM phosphorylation. MCM SUMOylation levels then decline as MCM phosphorylation levels rise, thus suggesting an inh... More

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