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OK/basigin expression on red blood cells varies between blood donors and correlates with binding of recombinant Plasmodium falciparum reticulocyte-binding protein homolog 5.

Transfusion. 2018; 
Ye L, Zhao F, Yang Q, Zhang J, Li Q, Wang C, Guo Z, Yang Y, Zhu Z.
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Cellular Analysis … performed After elution, the elute was analyzed by using a commercial His Tag ELISA detection kit (Genscript; Cat No L00436) Results are representative of three independent experiments that were performed in duplicates … Get A Quote

摘要

Recently, basigin (BSG), which carries OK antigens on red blood cells (RBCs), was reported to be the receptor of the Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRh5). BSG-PfRh5 is the only essential receptor-ligand pair in P. falciparum invasion that is known to date. However, the kind of OK/BSG polymorphism involved in the selection pressure caused by P. falciparum malaria has not been determined.,Blood samples were collected to detect the expression of OK/BSG. The coding region of PfRh5 was cloned and expressed. Enzyme-linked immunosorbent assay-based erythrocyte binding assay was used to measure the recombinant PfRh5 (rPfRh5) binding of RBCs with different OK/BSG expressions. Sequencing... More

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