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The protease activity of human ATG4B is regulated by reversible oxidative modification

Autophagy. 2020; 
Zheng X, Yang Z, Gu Q, Xia F, Fu Y, Liu P, Yin XM, Li M.
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Catalog Antibody Antibodies in this study used in western blot and immunofluorescence analysis were as follow: anti-ATG4B (Cell Signaling Technology, 5299S), anti-FLAG (Medical & Biological Laboratories, M185-32), anti-LC3B for western blot (Sigma, L7543), anti-LC3B for immunofluorescence (Medical & Biological Laboratories, PM036), anti-GST (GenScript, A00097), anti-GAPDH (Santa Cruz Biotechnology, sc-365062), anti-His (Thermo Fisher Scientific, MA1-135), anti-GSH (Santa Cruz Biotechnology, sc-52399), anti-NPM1 (Proteintech, 60096-1). Get A Quote

摘要

Macroautophagy/autophagy plays a pivotal role in cytoplasmic material recycling and metabolic turnover, in which ATG4B functions as a "scissor" for processing pro-LC3 and lipidated LC3 to drive the autophagy progress. Mounting evidence has demonstrated the tight connection between ROS and autophagy during various pathological situations. Coincidentally, several studies have shown that ATG4B is potentially regulated by redox modification, but the underlying molecular mechanism and its relationship with autophagy is ambiguous. In this study, we verified that ATG4B activity was definitely regulated in a reversible redox manner. We also determined that Cys292 and Cys361 are essential sites of ATG4B to form reversib... More

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