凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Molecular characterization of differences between the tomato immune receptors Fls3 and Fls2

biorxiv. 2020; 
Robyn Roberts,  Alexander E. Liu,  Lingwei Wan,  Annie M. Geiger,  Sarah R. Hind, Hernan G. Rosli,   Gregory B. Martin
Products/Services Used Details Operation
Molecular Biology Tools For the transiently-expressed proteins in N. benthamiana, total protein was extracted from Agroinfiltrated leaves, 5-10 μg was run on SDS-PAGE, blotted on PVDF membrane, and detected with anti-Myc antibodies (Genscript, #A00704 www.genscript.com) and ECL Plus chemiluminescent substrate (Thermo Fisher Scientific, www.thermofisher.com) as described in (Roberts et al., 2019a). Get A Quote

摘要

Plants mount defense responses by recognizing indications of pathogen invasion, including microbe-associated molecular patterns (MAMPs). Flagellin from the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) contains two MAMPs, flg22 and flgII-28, that are recognized by tomato receptors Flagellin sensing 2 (Fls2) and Flagellin sensing 3 (Fls3), respectively. It is unknown to what degree each receptor contributes to immunity and if they promote immune responses using the same molecular mechanisms. Characterization of CRISPR/Cas9-generated Fls2 and Fls3 tomato mutants revealed that the two receptors contribute equally to disease resistance both on the leaf surface and in the apoplast. However, striking... More

关键词

plant immunity; flagellin; PRR-triggered immunity; bacterial speck disease; tomato