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Multimeric antibodies with increased valency surpassing functional affinity and potency thresholds using novel formats

MAbs. 2020; 
Ami Miller, Stephen Carr, Terry Rabbitts, Hanif Ali
Products/Services Used Details Operation
Catalog Antibody … One hundred ul of detection antibody (anti-His-HRP, A7058, Sigma; or anti-Human-IgG HRP, 31410, Thermo Fisher Scientific; or Protein L HRP, M00098, Genscript) diluted in blocking … The absorbance at 450 nm was read using a CLARIOstar microplate reader (BMG Labtech) … Get A Quote

摘要

The success of therapeutic antibodies is largely attributed for their exquisite specificity, homogeneity, and functionality. There is, however, a need to engineer antibodies to extend and enhance their potency. One parameter is functional affinity augmentation, since antibodies matured in vivo have a natural affinity threshold. Generation of multivalent antibodies is one option capable of surpassing this affinity threshold through increased avidity. In this study, we present a novel platform consisting of an array of multivalent antibody formats, termed Quads, generated using the self-assembling tetramerization domain from p53. We demonstrate the versatility of this tetramerization domain by engineering anti-tu... More

关键词

Multivalent, antibody, avidity, bispecific, octavalent, tetravalent