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STAT1 participates in the induction of substance P expression in airway epithelial cells by respiratory syncytial virus

Exp Lung Res. 2020-11; 
Yu-Long Luo, Sheng Wang, Zhi-Xin Fang, Yi-Chu Nie, Li-Ting Zhang, Chu-Qin Huang, Li Long, Ke-Fang Lai
Products/Services Used Details Operation
… IFN-α (20 ng/ml) (Genscript, Z03003), STAT1 inhibitor fludarabine (1 μM) (Selleck, S1491 … and the control BEAS-2B cells were constructed by the Beyotime Institute of Biotechnology (China) with … All-in-One First-Strand cDNA Synthesis SuperMix kit for qPCR (TransGen Biotech Co …

摘要

objective: The regulation effect and mechanism of respiratory syncytial virus (RSV) infection on the expression of tachykinin substance P (SP) in airway epithelial cells was investigated. methods: The regulation of SP expression by RSV was investigated in the BEAS-2B airway epithelial cell line. RT-qPCR, immunofluorescence, and ELISA assay were used to examine the expression of the SP encoding gene , the intracellular SP protein expression, and the extracellular SP secretion. results: The mRNA expression of and the intracellular SP protein level in BEAS-2B cells were significantly enhanced by RSV infection with multiplicity of infection (MOI) values of both 1 and 0.1 at 48 hours post infection. Heat-inactiva... More

关键词

Airway epithelial cells, STAT1, respiratory syncytial virus, substance P, tachykinin precursor 1 gene