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Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Autophagy. 2021-11; 
Jia-Min Yan, Wen-Kang Zhang, Li-Na Yan, Yong-Jun Jiao, Chuan-Min Zhou, Xue-Jie Yu
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Proteins, Expression, Isolation and Analysis … , then anti-NP or anti-Gn antibody was purified using a protein A column (Genscript, L00464) and a Superdex 200 column (Cytiva, 28,989,335). … Areas containing cells were block mounted and thinly sliced using Leica EM UC7. Samples were imaged using a HITACHI HT7800 … Get A Quote

摘要

Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging negatively stranded enveloped RNA bunyavirus that causes SFTS with a high case fatality rate of up to 30%. Macroautophagy/autophagy is an evolutionarily conserved process involved in the maintenance of host homeostasis, which exhibits anti-viral or pro-viral responses in reaction to different viral challenges. However, the interaction between the bunyavirus SFTSV and the autophagic process is still largely unclear. By establishing various autophagy-deficient cell lines, we found that SFTSV triggered RB1CC1/FIP200-BECN1-ATG5-dependent classical autophagy flux. SFTSV nucleoprotein induced BECN1-dependent autophagy by disrupting the BECN1-BCL... More

关键词

Autophagy, bunyavirus, sftsv, viral assembly, viral egress