凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Ultrasensitive Detection of SARS-CoV-2 Spike Proteins Using the Thio-NAD Cycling Reaction: A Preliminary Study before Clinical Trials

Microorganisms. 2021-10; 
Yuta Kyosei, Mayuri Namba, Daiki Makioka, Ayumi Kokubun, Satoshi Watabe, Teruki Yoshimura, Tadahiro Sasaki, Tatsuo Shioda, Etsuro Ito
Products/Services Used Details Operation
Mammalian Expression … The recombinant antigen for SARS-CoV-2 spike S1 protein was obtained from GenScript (human cell source, 78.3 kDa, Cat #Z03501). Alkaline phosphatase (ALP) and nicotinamide adenine dinucleotide (NADH) were purchased from Roche. The thio-NAD was obtained from … Get A Quote

摘要

To help control the global pandemic of coronavirus disease 2019 (COVID-19), we developed a diagnostic method targeting the spike protein of the virus that causes the infection, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We applied an ultrasensitive method by combining a sandwich enzyme-linked immunosorbent assay (ELISA) and the thio-nicotinamide adenine dinucleotide (thio-NAD) cycling reaction to quantify spike S1 proteins. The limit of detection (LOD) was 2.62 × 10 moles/assay for recombinant S1 proteins and 2.6 × 10 RNA copies/assay for ultraviolet B-inactivated viruses. We have already shown that the ultrasensitive ELISA for nucleocapsid proteins can detect ultraviolet B-inactivated viru... More

关键词

COVID-19, SARS-CoV-2, antigen test, spike protein, thio-NAD cycling, ultrasensitive ELISA