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Simultaneous evaluation of antibodies that inhibit SARS-CoV-2 variants via multiplex assay

JCI Insight. 2021-08; 
Ester Lopez, Ebene R Haycroft, Amy Adair, Francesca L Mordant, Matthew T O'Neill, Phillip Pymm, Samuel J Redmond, Wen Shi Lee, Nicholas A Gherardin, Adam K Wheatley, Jennifer A Juno, Kevin J Selva, Samantha K Davis, Samantha L Grimley, Leigh Harty, Damian Fj Purcell, Kanta Subbarao, Dale I Godfrey, Stephen J Kent, Wai-Hong Tham, Amy W Chung
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Recombinant Antibody Expression … using identical protocols as control proteins. Coupling efficiency of the recombinant RBD was assessed using an anti-His Tag antibody (A00174, GenScript Corporation). A complete list of the magnetic bead regions and respective RBD variants is in Supplemental Table 3. … Get A Quote

摘要

The SARS-CoV-2 receptor binding domain (RBD) is both the principal target of neutralizing antibodies and one of the most rapidly evolving domains, which can result in the emergence of immune escape mutations, limiting the effectiveness of vaccines and antibody therapeutics. To facilitate surveillance, we developed a rapid, high-throughput, multiplex assay able to assess the inhibitory response of antibodies to 24 RBD natural variants simultaneously. We demonstrate how this assay can be implemented as a rapid surrogate assay for functional cell-based serological methods to measure the SARS-CoV-2 neutralizing capacity of antibodies at the angiotensin-converting enzyme 2-RBD (ACE2-RBD) interface. We describe the e... More

关键词

COVID-19, Immunoglobulins