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Differentiation and characterization of human iPSC-derived vascular endothelial cells under physiological shear stress

STAR Protoc. 2021-03; 
Nadia O Abutaleb, George A Truskey
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Recombinant Proteins … Note: Our flow chamber which is pictured throughout the protocol has a metal bottom with a glass window in the center to enable visualization of the slide, and an acrylic top … Heparin, Sigma-Aldrich, H3149. Human VEGF165, GenScript, Z02689 … Get A Quote

摘要

Induced pluripotent stem cells (iPSCs) offer a potentially unlimited source to generate endothelial cells (ECs) for numerous applications. Here, we describe a 7-day protocol to differentiate up to 55 million vascular endothelial cells (viECs) from 3.5 million human iPSCs using small molecules to regulate specific transcription factors. We also describe a parallel-plate flow chamber system to study EC behavior under physiological shear stress. For complete details on the use and execution of this protocol, please refer to Atchison et al. (2020).

关键词

Biophysics, Cell Differentiation, Cell culture, Stem Cells