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NIX Mediates Mitophagy in Spinal Cord Injury in Rats by Interacting with LC3

Cell Mol Neurobiol. 2021-03; 
Piming Nie, Honggang Wang, Datang Yu, Hongchen Wu, Bing Ni, Jiming Kong, Zhengfeng Zhang
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Polyclonal Antibody Services … The method of equal quality and equal quantity was adopted when the proteins (10 μl, 30 μg) were separated by electrophoresis on 4–20% or 12% SurePAGE™ Bis–Tris Protein Gels (GenScript, Nangjing, China, M00656/M0066) and transferred onto the polyvinylidene fluoride … Get A Quote

摘要

Excessive mitophagy plays a role in neuronal death in spinal cord injury (SCI), its molecular regulation remains largely unknown. The present study aims to determine the role of NIX, a member of a unique subfamily of death-inducing mitochondrial proteins, in the regulation of mitophagy in SCI. Here we show that NIX is highly upregulated in SCI and hypoxia, and localized to mitochondria. The mitochondria-bound NIX interacts with autophagosome-localized LC3 (Microtubule-associated protein 1 light chain 3) to form a mitochondria-NIX-LC3-autophagosome complex, resulting in excessive mitophagy in SCI. Downregulation of NIX by RNA interference restores the function of mitochondria in spinal cord neurons under hypoxia... More

关键词

Hypoxia, LC3, Mitophagy, NIX, Spinal cord injury