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Structure of a TOC-TIC supercomplex spanning two chloroplast envelope membranes

Cell. 2022-11; 
Zeyu Jin , Li Wan , Yuqi Zhang , Xuecheng Li , Yong Cao , Haobin Liu , Shengyao Fan , Du Cao , Zhengmao Wang , Xiaobo Li , Junmin Pan , Meng-Qiu Dong , Jianping Wu , Zhen Yan
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摘要

The TOC and TIC complexes are essential translocons that facilitate the import of the nuclear genome-encoded preproteins across the two envelope membranes of chloroplast, but their exact molecular identities and assembly remain unclear. Here, we report a cryoelectron microscopy structure of TOC-TIC supercomplex from Chlamydomonas, containing a total of 14 identified components. The preprotein-conducting pore of TOC is a hybrid β-barrel co-assembled by Toc120 and Toc75, while the potential translocation path of TIC is formed by transmembrane helices from Tic20 and YlmG, rather than a classic model of Tic110. A rigid intermembrane space (IMS) scaffold bridges two chloroplast membranes, and a large hydrophilic cl... More

关键词

Chlamydomonas reinhardtii; TOC-TIC supercomplex; chloroplast; cryo-EM structure; protein import; translocon.