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Asymmetric Stratification-Induced Polarity Loss and Coordinated Individual Cell Movements Drive Directional Migration of Vertebrate Epithelium

Cell Rep. 2022-10; 
Yunzhe Lu , Ruolan Deng , Huanyang You , Yishu Xu , Christopher Antos , Jianlong Sun , Ophir D Klein , Pengfei Lu
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Recombinant Proteins Branching assay was done as described previously (Ewald et al., 2008). FGF2 (2.5nM, GenScript, #Z03116–50), or FGF10 (2.5nM, GenScript, #Z03155–50) were used in the 5-day culture. Get A Quote

摘要

Collective migration is essential for development, wound repair, and cancer metastasis. For most collective systems, "leader cells" determine both the direction and the power of the migration. It has remained unclear, however, how the highly polarized vertebrate epithelium migrates directionally during branching morphogenesis. We show here that, unlike in other systems, front-rear polarity of the mammary epithelium is set up by preferential cell proliferation in the front in response to the FGF10 gradient. This leads to frontal stratification, loss of apicobasal polarity, and leader cell formation. Leader cells are a dynamic population and move faster and more directionally toward the FGF10 signal than do follo... More

关键词

FGF gradient; apicobasal polarity; branching morphogenesis; cell polarity; chemotaxis; collective migration; epithelial polarity; epithelial stratification; epithelial-mesenchymal transition; front-rear polarity.