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Development of a universal antibiotic resistance screening reporter for improving efficiency of cytosine and adenine base editing

J Biol Chem. 2022-06; 
Lixia Ma, Jiani Xing, Qian Li, Zhiying Zhang, Kun Xu
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Proteins, Expression, Isolation and Analysis … Protein samples were resolved by electrophoresis in a 12% SDS-PAGE gel (Genscript, China, M00667) and were transferred to a PVDF membrane in transfer buffer (Genscript, China, … Get A Quote

摘要

Base editing has emerged as a revolutionary technology for single nucleotide modifications. The cytosine and adenine base editors (CBEs and ABEs) have demonstrated great potential in clinical and fundamental research. However, screening and isolating target-edited cells remains challenging. In the current study, we developed a universal Adenine and Cytosine Base-Editing Antibiotic Resistance Screening Reporter (ACBE-ARSR) for improving the editing efficiency. To develop the reporter, the CBE-ARSR was first constructed and shown to be capable of enriching cells for those that had undergone CBE editing activity. Then, the ACBE-ARSR was constructed and was further validated in the editing assays by four different ... More

关键词

adenine base editor, base-editing activity, cytosine base editor, screening, universal reporter