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Residues L55 and W69 of Tva Mediate Entry of Subgroup A Avian Leukosis Virus

J Virol. 2022-09; 
Yuntong Chen, Suyan Wang, Xinyi Li, Mengmeng Yu, Peng Liu, Lingzhai Meng, Ru Guo, Xiaoyan Feng, Aijing Liu, Xiaole Qi, Kai Li, Li Gao, Qing Pan, Yanping Zhang, Changjun Liu, Hongyu Cui, Xiaomei Wang, Yulong Gao
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Proteins, Expression, Isolation and Analysis … in the trimeric glycoprotein structure, exposing the TM glycoprotein domains responsible for … using protein A affinity matrix beads (L00210; GenScript) according to the manufacturer’s … Get A Quote

摘要

The receptor of the subgroup A avian leukosis virus (ALV-A) in chicken is Tva, which is the homologous protein of human CD320 (huCD320), contains a low-density lipoprotein (LDL-A) module and is involved in the uptake of transcobalamin bound vitamin B/cobalamin (Cbl). To map the functional determinants of Tva responsible for ALV-A receptor activity, a series of chimeric receptors were created by swapping the LDL-A module fragments between huCD320 and Tva. These chimeric receptors were then used for virus entry and binding assays to map the minimal ALV-A functional domain of Tva. The results showed that Tva residues 49 to 71 constituted the minimal functional domain that directly interacted with the ALV-A gp85 pr... More

关键词

receptors, subgroup A avian leukosis virus, virus entry