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Evaluating blood-brain barrier disruption and infarction volume concurrently in rats subjected to ischemic stroke using an optical imaging system

J Neurosci Methods. 2022-05; 
Yao He, Yingqian Zhang, Wanxin Li, Qinxi Li, Bangcheng Zhao, Xun Tang, Daojun Chen, Ting Zhang, Tao Zhang, Zhihui Zhong
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Proteins, Expression, Isolation and Analysis … right cerebral cortex in the Sham group (n = 3) and MCAO group (n = 3) were taken for Western … in 12% precast gels (M00669, GenScript Biotech, China), transferred onto polyvinylidene … Get A Quote

摘要

background: Blood-brain barrier (BBB) disruption is pivotal in the pathophysiological process of ischemic stroke and is often measured in rodent stroke studies. Traditionally, rodent BBB permeability increase is determined by measuring cerebral leakage of certain dyes such as Evans Blue or sodium fluorescein (NaFL). However, due to the special processing of samples for BBB permeability measurement, they cannot be used afterward for determining other essential parameters such as cerebral infarction volume. Therefore, using different batches of animals for assessing BBB permeability and infarction volume is typical. However, this would limit the stroke study's statistical power and scientific value while hinderin... More

关键词

Animal welfare, BBB, Cerebral infarction volume, MCAO, Optical In Vivo Imaging System