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Transcriptional and metabolic remodeling in clear cell renal cell carcinoma caused by ATF4 activation and the integrated stress response (ISR)

Mol Carcinog. 2022-06; 
Johannes C van der Mijn, Qiuying Chen, Kristian B Laursen, Francesca Khani, Xiaofei Wang, Princesca Dorsaint, Andrea Sboner, Steven S Gross, David M Nanus, Lorraine J Gudas
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Proteins, Expression, Isolation and Analysis … gel electrophoresis gel in MOPS buffer (GenScript #M00656) and then blotted … (n = 60 genes) using the transcriptomics data of patients with ccRCC versus normal kidney specimens (n … Get A Quote

摘要

Research has shown extensive metabolic remodeling in clear cell renal cell carcinoma (ccRCC), with increased glutathione (GSH) levels. We hypothesized that activating transcription factor-4 (ATF4) and the integrated stress response (ISR) induce a metabolic shift, including increased GSH accumulation, and that Vitamin A deficiency (VAD), found in ccRCCs, can also activate ATF4 signaling in the kidney. To determine the role of ATF4, we used publicly available RNA sequencing (RNA-seq) data sets from The Cancer Genomics Atlas. Subsequently, we performed RNA-seq and liquid chromatography-mass spectrometry-based metabolomics analysis of the murine TRAnsgenic Cancer of the Kidney (TRACK) model for early-stage ccRCC. T... More

关键词

HIF, glutathione, kidney cancer, nutrition, retinoids