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An inducible gene from glycoside hydrolase one family of decreases larval survival when feeding on host plant

Front Physiol. 2022-10; 
Wei Chen, Yuhong Dong, Ling Zheng, Yingfang Lai, Feifei Li, Li Zhou, Beibei Wang, Minsheng You, Weiyi He
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摘要

Glycoside hydrolase family 1 (GH1) members exhibit a broad substrate spectrum and play important roles in insect-plant interactions, such as the defensive β-glucosidase and β-thioglucosidase (so-called myrosinase). However, knowledge about the expression profiling and function of glycoside hydrolase family 1 members in a specialist pest of crucifers is still limited. In this study, 13 putative glycoside hydrolase family 1 members of were identified based on the sequence characteristics, while no myrosinase activity was detectable in using gas chromatography-mass spectrometry (GC-MS). Expression profiling of these glycoside hydrolase family 1 members identified the midgut-specific gene that is induced by h... More

关键词

CRISPR/Cas9, Plutella xylostella, insect myrosinase, plant defense, toxifying gene