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Potential of peptide-engineered exosomes with overexpressed miR-92b-3p in anti-angiogenic therapy of ovarian cancer

Clin Transl Med. 2021-05; 
Jiaying Wang, Conghui Wang, Yang Li, Mingyue Li, Tingjia Zhu, Zhangjin Shen, Hui Wang, Weiguo Lv, Xinyu Wang, Xiaodong Cheng, Xing Xie
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Proteins, Expression, Isolation and Analysis The cells or exosomes were lysed with RIPA (R0010, Solarbio), and protein samples with equivalent amount were separated by SurePAGE gels (M00665, GenScript) and transferred onto 0.2‐μm PVDF membranes (1620177, Bio‐Rad) by the eBlot L1 protein transfer system (L00686C, GenScript).  Get A Quote

摘要

background: Exosomal microRNA (miRNA) as a mediator of intercellular communication plays an essential part in tumor-relevant angiogenesis. Therapy against angiogenesis has been demonstrated to have a remarkable antitumor efficacy in various malignancies, but not as expected in ovarian cancer. methods: Exosomes were isolated by ultracentrifugation. Exosomal miRNA sequencing and gene function experiments were used to identify the differential expressed miRNAs in exosomes and their mRNA targets. SKOV3 cell line that stably overexpressed miR-92b-3p was constructed by lentivirus. In vitro, angiogenesis was analyzed by tube formation assay and migration assay. The angiogenic and antitumor effects in vivo were assesse... More

关键词

Apatinib, anti-angiogenesis, engineered exosomes, miR-92b-3p, ovarian cancer