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The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity

J Cell Sci. 2021-12; 
Kari H Ecklund, Megan E Bailey, Kelly A Kossen, Carsten K Dietvorst, Charles L Asbury, Steven M Markus
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摘要

Dynein motors move the mitotic spindle to the cell division plane in many cell types, including in budding yeast, in which dynein is assisted by numerous factors including the microtubule-associated protein (MAP) She1. Evidence suggests that She1 plays a role in polarizing dynein-mediated spindle movements toward the daughter cell; however, how She1 performs this function is unknown. We find that She1 assists dynein in maintaining the spindle in close proximity to the bud neck, such that, at anaphase onset, the chromosomes are segregated to mother and daughter cells. She1 does so by attenuating the initiation of dynein-mediated spindle movements within the mother cell, thus ensuring such movements are polarized... More

关键词

Dynactin, Dynein, Microtubule-associated proteins, She1, Spindle positioning