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Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling

Nat Commun. 2021-01; 
Carleen Klumpp-Thomas, Heather Kalish, Matthew Drew, Sally Hunsberger, Kelly Snead, Michael P Fay, Jennifer Mehalko, Anandakumar Shunmugavel, Vanessa Wall, Peter Frank, John-Paul Denson, Min Hong, Gulcin Gulten, Simon Messing, Jennifer Hicks, Sam Michael, William Gillette, Matthew D Hall, Matthew J Memoli, Dominic Esposito, Kaitlyn Sadtler
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Mammalian Expression Healthy seronegative whole blood collected in EDTA-coated tubes was spiked with commercially available human chimeric anti-RBD IgG and IgM antibodies (GenScript, Cat# A02038, A02046).  Get A Quote

摘要

The extent of SARS-CoV-2 infection throughout the United States population is currently unknown. High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities. Validation of this protocol is performed using traditionally collected serum as well as dried blood on mail-in blood sampling kits. Archival (pre-2019) samples are used as negative controls, and convalescent, PCR-diagnosed COVID-19 patient samples serve as positive controls. Using this proto... More

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