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Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight

J Pineal Res. 2021-12; 
Jingru Guo, Yujing Bai, Yunxie Wei, Yabin Dong, Hongqiu Zeng, Russel J Reiter, Haitao Shi
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Proteins, Expression, Isolation and Analysis … A/G Magbeads (L00277, GenScript, Nanjing, China), and then Myc antibodies (AM926, Beyotime, Shanghai, China) were added into the fusion protein overnight for incubation. The … Get A Quote

摘要

Melatonin is widely involved in plant disease resistance through modulation of immune responses. Pathogenesis-related (PR) proteins play important roles in plant immune responses. However, the direct association between melatonin biosynthetic enzyme and PR protein remains elusive in plants. In this study, we found that N-acetylserotonin O-methyltransferase 2 (MeASMT2) physically interacted with MePR1 in vitro and in vivo, thereby promoting the anti-bacterial activity of MePR1 against Xanthomonas axonopodis pv. manihotis (Xam). Consistently, MeASMT2 improved the effect of MePR1 on positively regulating cassava disease resistance. In addition, we found that type 2C protein phosphatase 1 (MePP2C1) interacted with ... More

关键词

cassava bacterial blight, melatonin biosynthetic enzyme, pathogenesis-related protein, protein-protein interaction, type 2C protein phosphatase