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Glycoengineering directs de novo biomanufacturing of UPEC O21 O-antigen polysaccharide based glycoprotein

Int J Biol Macromol .. 2023-09; 
Yuhui Wang , Andrei V Perepelov , Sof'ya N Senchenkova , Gege Lu , Xiaohan Wang , Guozhen Ma , Qian Yang , Jian Yuan , Yanling Wang , Lijie Xie , Xiaolong Jiang , Jingliang Qin , Dan Liu , Miaomiao Liu , Di Huang , Bin Liu
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Proteins, Expression, Isolation and Analysis Purified glycoproteins were boiled for 5–10 min and then loaded onto 4–12 % SurePAGE™ Bis-Tris gels for electrophoresis (#M00652, GenScript Corporation, China). Get A Quote

摘要

Glycoproteins, in which polysaccharides are usually attached to proteins, are an important class of biomolecules that are widely used as therapeutic agents in clinical treatments for decades. Uropathogenic Escherichia coli (UPEC) O21 has been identified as a serogroup that induces urinary tract infections, with a global increasing number among women and young children. Therefore, there is an urgent need to establish protective vaccines against UPEC infection. Herein, we engineered non-pathogenic E. coli MG1655 to achieve robust, cost-effective de novo biosynthesis of O21 O-antigen polysaccharide-based glycoprotein against UPEC O21. Specifically, this glycoengineered E. coli MG1655 was manipulated for high-effic... More

关键词

Glycoprotein; O21 O-antigen gene cluster; Polysaccharides; Protein glycosylation; UDP-sugar precursors; Urinary tract infections (UTI).