凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A Biosafety Study of Human Umbilical Cord Blood Mononuclear Cells Transduced with Adenoviral Vector Carrying Human Vascular Endothelial Growth Factor cDNA In Vitro

Biomedicines .. 2023-07; 
Ilnur I Salafutdinov , Dilara Z Gatina , Maria I Markelova , Ekaterina E Garanina , Sergey Yu Malanin , Ilnaz M Gazizov , Andrei A Izmailov , Albert A Rizvanov , Rustem R Islamov , András Palotás , Zufar Z Safiullov
Products/Services Used Details Operation
Catalog Antibody Afterwards, the PVDF membranes were incubated with Abs against VEGF (Sigma,Saint Louis, MO, USA, V6627, 1:1000) and β-actin (Genscript, Piscataway, NJ, USA, A00730-40, 1:3000) overnight at 4 ◦C. Horseradish peroxidase conjugated Abs were used to visualize the target proteins [59]. Get A Quote

摘要

The biosafety of gene therapy remains a crucial issue for both the direct and cell-mediated delivery of recombinant cDNA encoding biologically active molecules for the pathogenetic correction of congenital or acquired disorders. The diversity of vector systems and cell carriers for the delivery of therapeutic genes revealed the difficulty of developing and implementing a safe and effective drug containing artificial genetic material for the treatment of human diseases in practical medicine. Therefore, in this study we assessed changes in the transcriptome and secretome of umbilical cord blood mononuclear cells (UCB-MCs) genetically modified using adenoviral vector (Ad5) carrying cDNA encoding human vascular end... More

关键词

biosafety; cord blood stem cell transplantation; genetic therapy; genetic vectors; secretome; transcriptome; vascular endothelial growth factor A.