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Lipopolysaccharides from a Shiraia fruiting body-associated bacterium elicit host fungal hypocrellin A biosynthesis through nitric oxide generation

Carbohydr Polym . . 2024-01; 
Xin Ping Li , Wen Hao Shen , Lu Lu Zhou , Qun Yan Huang , Rui Peng Cong , Li Ping Zheng , Jian Wen Wang
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摘要

Hypocrellin A (HA) is an excellent perylenequinone photosensitizer from Shiraia fruiting bodies. A dominant bacterium Pseudomonas fulva SB1 in the fruiting body was found to promote HA biosynthesis. The bacterial LPS were purified and the O-specific polysaccharide (OPS) consisted of rhamnose (Rha), galactose (Gal) and N-acetyl-galactosamine (GalNAc) with an average molecular weight of 282.8 kDa. Although the OPS composing of Rhap and Galp backbone showed elicitation capability on fungal HA accumulation, the highest HA production (303.76 mg/L) was achieved by LPS treatment at 20 μg/mL on day 3 of the mycelium culture. The generation of nitric oxide (NO) in Shiraia mycelia was triggered by LPS, which was partial... More

关键词

Hypocrellin; Lipopolysaccharides; Nitric oxide; O-specific polysaccharide; Pseudomonas fulva SB1; Shiraia.