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Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model.

J Tissue Eng Regen Med.. 2012-08; 
Fiona Wegman, Ruth E. Geuze, Yvonne J. van der Helm, F. Cumhur Öner, Wouter J.A. Dhert, Jacqueline Alblas. Department of Orthopaedics, University Medical Centre Utrecht, The Netherlands
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摘要

In the field of bone regeneration, BMP-2 is considered one of the most important growth factors because of its strong osteogenic activity, and is therefore extensively used in clinical practice. However, the short half-life of BMP-2 protein necessitates the use of supraphysiological doses, leading to severe side-effects. This study investigated the efficiency of bone formation at ectopic and orthotopic sites as a result of a low-cost, prolonged presence of BMP-2 in a large animal model. Constructs consisting of alginate hydrogel and BMP-2 cDNA, together acting as a non-viral gene-activated matrix, were combined with goat multipotent stromal cells (gMSCs) and implanted in spinal cassettes or, together with ceram... More

关键词

plasmid DNA; bone tissue engineering; alginate hydrogel; gene therapy; bone morphogenetic protein; gene-activated matrix