位点扫描库是多肽序列优化的一个重要工具。在目的位点上替换全部的天然氨基酸，每条多肽替换一次，可用来鉴别活性较佳的多肽序列。常用在复杂的蛋白混合物中鉴定T细胞抗原表位，或用相互依赖的子位点来定位底物，以减少合成和筛选工作量。
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